abstract：:In this paper the development and validation of a method for the analysis of THC-COOH, THC, THC-OH, CBD and CBN in their total form in urine by LC-MS/MS is presented. Tandem hydrolysis, i.e. enzymatic and basic, has been found optimal for the simultaneous analysis of the selected analytes in urine: basic hydrolysis is...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Montesano C,Sergi M,Odoardi S,Simeoni MC,Compagnone D,Curini R

更新日期：2014-03-01 00:00:00

abstract：:In this study, a selective sample pretreatment procedure combing surface molecularly imprinted polymers and microextraction in packed syringe (SMIPs-MEPS) was developed for the analysis of clenbuterol (CLB) from pork samples. SMIPs for CLB were synthesized on silica gel particles through a sol-gel process. A series of...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Du W,Lei C,Zhang S,Bai G,Zhou H,Sun M,Fu Q,Chang C

更新日期：2014-03-01 00:00:00

abstract：:The present work describes the development and validation of rapid, sensitive and accurate liquid chromatography method, coupled with tandem mass spectrometry detection, for quantification of tranexamic acid in human plasma using isotopically labeled internal standard (IS). A one-step plasma protein precipitation was ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Delavenne X,Montbel A,Hodin S,Zufferey P,Basset T

更新日期：2014-03-01 00:00:00

abstract：:Sweat is a biofluid with present scant use as clinical sample. This review tries to demonstrate the advantages of sweat over other biofluids such as blood or urine for routine clinical analyses and the potential when related to metabolomics. With this aim, critical discussion of sweat samplers and equipment for analys...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mena-Bravo A,Luque de Castro MD

更新日期：2014-03-01 00:00:00

abstract：:Natural and synthetic opioids have efficient analgesic activity but can also be addictive. Thus, the determination of opioids and their metabolites in biological specimens is of interest in clinical and forensic toxicology laboratories. The analysis of drugs in hair provides valuable information on previous chronic dr...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Kim J,Ji D,Kang S,Park M,Yang W,Kim E,Choi H,Lee S

更新日期：2014-02-01 00:00:00

abstract：:Non covalent grafting of proteins on affinity phases is a very common approach for isolation, purification and re-concentration of tagged proteins. Many biophysical studies are conducted on these grafted proteins (surface plasmon resonance, quartz crystal microbalance, etc.) showing that the integrity and function of ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Viéville JM,Charbonnier S,Eberling P,Starck JP,Delsuc MA

更新日期：2014-02-01 00:00:00

abstract：:This study assessed a general method of quantitative nuclear magnetic resonance (qNMR) for the calibration of atropine sulfate (Active Pharmaceutical Ingredient, API) as reference standard. The spectra were acquired in D2O using maleic acid as the internal standard. Conformational behaviors of tropane ring were observ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Shen S,Yao J,Shi Y

更新日期：2014-02-01 00:00:00

abstract：:A simple, precise, economic and minimally operator-dependent method was developed under green analytical chemistry principles, for the simultaneous construction of the dissolution curves of a pharmaceutical association in short time and without employing organic solvents, allowing important savings of labor and resour...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Calvo NL,Maggio RM,Kaufman TS

更新日期：2014-02-01 00:00:00

abstract：:A selective and sensitive method utilizing gas chromatography-mass spectrometry was developed for simultaneous determination of cinnamaldehyde, cinnamyl alcohol, and methyl cinnamate in rat plasma. Cinnamaldehyde and cinnamyl alcohol can inter-convert to one another in rats, thus simultaneous quantifying both analytes...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhao H,Xie Y,Yang Q,Cao Y,Tu H,Cao W,Wang S

更新日期：2014-02-01 00:00:00

abstract：:A reversed-phase high performance liquid chromatographic (LC), tandem mass spectrometry (MS/MS) assay for the determination of tenofovir (TFV) and emtricitabine (FTC) in dried blood spots (DBS) from human whole blood was developed and validated. Whole blood samples were spotted, dried, and a 3mm punch was extracted wi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zheng JH,Guida LA,Rower C,Castillo-Mancilla J,Meditz A,Klein B,Kerr BJ,Langness J,Bushman L,Kiser J,Anderson PL

更新日期：2014-01-01 00:00:00

abstract：:2-Phosphonomethyl pentanedioic acid (2-PMPA) is a potent and selective inhibitor of glutamate carboxypeptidase-II, an enzyme which catabolizes the abundant neuropeptide N-acetyl-aspartyl-glutamate (NAAG) to N-acetylaspartate (NAA) and glutamate. 2-PMPA demonstrates robust efficacy in numerous animal models of neurolog...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Rais R,Rojas C,Wozniak K,Wu Y,Zhao M,Tsukamoto T,Rudek MA,Slusher BS

更新日期：2014-01-01 00:00:00

abstract：:The phytochemical research based on ethnopharmacology is considered an effective approach in the discovery of novel chemicals entities with potential as drug leads. Plants/plant extracts/decoctions, used by folklore traditions for treating several diseases, represent a source of chemical entities but no information ar...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Brusotti G,Cesari I,Dentamaro A,Caccialanza G,Massolini G

更新日期：2014-01-01 00:00:00

abstract：:Extracts of Acacia rigidula leaves are used in weight-loss products sold in vitamin shops and over the internet with little or no published data about their potential biological effects. In our chemical investigations on authenticated A. rigidula plant material, we established a rapid and sensitive LC-MS/MS method for...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Pawar RS,Grundel E,Fardin-Kia AR,Rader JI

更新日期：2014-01-01 00:00:00

abstract：:CPZEN-45 is a new drug candidate being considered for the treatment of tuberculosis (TB). The aim of this study was to develop and validate a reverse-phase high-performance liquid chromatographic (HPLC) method suitable to determine CPZEN-45 concentrations in biological samples. CPZEN-45 was extracted from biological f...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hanif SN,Hickey AJ,Garcia-Contreras L

更新日期：2014-01-01 00:00:00

abstract：:Iron is a challenging element due to its high background in various matrixes including blood, tissues even in the air and it is urgent to develop a method for the accurate determination of iron in bio-samples. After optimization of mass spectrometric conditions using collision cell technology and compensating for inte...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhao D,Zhang Y,Wang Y,Xu C,Dong C,Li C,Ren S,Zhang W,Lu Y,Dai Y,Chen X

更新日期：2014-01-01 00:00:00

abstract：:Banxia Xiexin decoction (BXD) is an effective Chinese Medicinal Prescription in treating gastroenteritis diseases. In this study an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to separate and determine 18 major active ingredients of BXD in order to guarantee ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Wang Y,Xu R,Xiao J,Zhang J,Wang X,An R,Ma Y

更新日期：2014-01-01 00:00:00

abstract：:Structured nanoparticles (NPs) with controlled size distribution and novel physicochemical features present fundamental advantages as drug delivery systems with respect to bulk drugs. NPs can transport and release drugs to target sites with high efficiency and limited side effects. Regulatory institutions such as the ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zattoni A,Roda B,Borghi F,Marassi V,Reschiglian P

更新日期：2014-01-01 00:00:00

abstract：:Miniaturization of analytical procedures through microchips, lab-on-a-chip or micro total analysis systems is one of the most recent trends in chemical and biological analysis. These systems are designed to perform all the steps in an analytical procedure, with the advantages of low sample and reagent consumption, fas...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Mirasoli M,Guardigli M,Michelini E,Roda A

更新日期：2014-01-01 00:00:00

abstract：:A liquid chromatographic method was developed for the simultaneous separation of curcumin, β-arteether, tetrahydrocurcumin and dihydroartemisinin based on the design of experiments and the design space methodology. The influence of the percentage of organic modifier, flow rate of the mobile phase and column temperatur...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Memvanga PB,Mbinze JK,Rozet E,Hubert P,Préat V,Marini RD

更新日期：2014-01-01 00:00:00

abstract：:Chlorogenic acids and derivatives like phenolic acids are potentially bioactive phenolics, which are commonly found in many foods. Once absorbed, chlorogenic and phenolic acids are highly metabolized by the intestine and the liver, producing glucuronidated and/or sulphated compounds. These metabolites were analyzed in...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Marmet C,Actis-Goretta L,Renouf M,Giuffrida F

更新日期：2014-01-01 00:00:00

abstract：:This paper describes a new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method for the analysis of three stanozolol metabolites (16β-hydroxystanozolol, 3'-hydroxystanozolol, and 4β-hydroxystanozolol) in urines of animal origins. The solid-phase extraction (SPE) clean-up procedure was optimized to reduce ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Tölgyesi Á,Sharma VK,Fekete J

更新日期：2014-01-01 00:00:00

abstract：:An automated on-line SPE and innovative fast polarity switch bioanalysis method employing dual-gradient liquid chromatography (DGLC) coupled with mass spectrometry (DGLC-MS/MS) was established and validated for the simultaneous determination of ferulic acid, ligustrazine and ligustilide in rat plasma after administrat...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zeng M,Zhang J,Yang Y,Jin Y,Xiao W,Wang Z,Ding G,Yan R

更新日期：2014-01-01 00:00:00

abstract：:A micellar electrokinetic chromatography (MEKC) method was developed for the determination of three anti-vomiting drugs (aprepitant, dexamethasone and ondansetron) in pharmaceutical formulations. The method was optimized using a central composite design (CCD). Four main factors (borate buffer concentration, pH, methan...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Bourdon F,Lecoeur M,Duhaut M,Odou P,Vaccher C,Foulon C

更新日期：2013-12-01 00:00:00

abstract：:A comprehensive study of fragmentation pathways of 15-membered azalide macrocycles using electrospray ionisation with multistage mass spectrometry (ESI-MS(n)) is presented in this work. Hydrogen/deuterium (H/D) exchange experiments and high-resolution mass spectrometry were used to investigate the proposed fragmentati...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gembarovski D,Marković VG,Galić N

更新日期：2013-12-01 00:00:00

abstract：:This review describes an epigrammatic impression of the recent trends in analytical perspectives of degradation and impurities profiling of pharmaceuticals including active pharmaceutical ingredient (API) as well as drug products during 2008-2012. These recent trends in forced degradation and impurity profiling were d...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Jain D,Basniwal PK

更新日期：2013-12-01 00:00:00

abstract：:A reliable method based on high performance liquid chromatography-diode array detector-electrospray ionization-ion trap-time of flight-mass spectrometry (HPLC-DAD-ESI-IT-TOF-MS) was developed for the identification of phenolic acids and flavonoids in Apocynum venetum L. leaves and its adulterant, Pocynum hendersonii (...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： An H,Wang H,Lan Y,Hashi Y,Chen S

更新日期：2013-11-01 00:00:00

abstract：:A sensitive and rapid ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method has been developed for the determination of 21-hydroxy deflazacort in human plasma using betamethasone as the internal standard (IS). After solid-phase extraction from 100 μL human plasma, the analyte and IS wer...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Patel DP,Sharma P,Patel BM,Sanyal M,Singhal P,Shrivastav PS

更新日期：2013-11-01 00:00:00

abstract：:A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) assay was developed and qualified for analyzing 4β-hydroxycholesterol and cholesterol in 5 μl of human and mouse plasma. Stable isotope-labeled d7-analogs of both analytes were used as internal standards and 4.2% (w/v) human serum ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Xu Y,Yuan Y,Smith L,Edom R,Weng N,Mamidi R,Silva J,Evans DC,Lim HK

更新日期：2013-11-01 00:00:00

abstract：:The evaluation of plasmatic galactosaminoglycans, dermatan sulfate (DS) and chondroitin sulfate (CS) can be helpful in the early identification of MPS patients, also considering that primary storage of one type of GAG can lead to secondary accumulation of other lysosomal substrates. We explore the possibility to deter...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Volpi N,Maccari F,Galeotti F,Zampini L,Santoro L,Padella L,Galeazzi T,Gabrielli O,Coppa GV

更新日期：2013-11-01 00:00:00

abstract：:There is increasing development of pegylated proteins as clinical products for therapeutic interventions in human disease. Quantification of such products relies on appropriately calibrated traditional methods, including reverse phase-high performance liquid chromatography (RP-HPLC). However, currently available pharm...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： El Ghazaly M,Meager A,Zikry H,Ebaed M,Shaker S,Mueller F,Rohde J

更新日期：2013-10-01 00:00:00

abstract：:Galloyl- and caffeoylquinic acids are among the most important pharmacological active groups of natural compounds. This study describes a pre-step in isolation of some selected representatives of these groups from biological samples. A selective solid-phase extraction (SPE) method for these compounds may help assign c...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Hussain S,Schönbichler SA,Güzel Y,Sonderegger H,Abel G,Rainer M,Huck CW,Bonn GK

更新日期：2013-10-01 00:00:00

abstract：:Isoflavonoids are widespread constituents in medical plants especially in legumes (Fabaceae), but occur in other different plant families as well (Rosaceae, Iridaceae, Amaranthaceae). Their antioxidant, estrogen-like, anti-inflammatory and analgesic effects make them promising compounds in therapy of important disorde...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Daruházi AE,Kiss T,Vecsernyés M,Szente L,Szőke E,Lemberkovics E

更新日期：2013-10-01 00:00:00

abstract：:Hemp seed soft gel capsule (HSSGC) is a modernised dosage form that is derived from a traditional Chinese patent medicine, Hemp Seed Pills (HSP). Two dosage forms claim the same therapeutic effects; however, their chemical components and chemical equivalency are unclear. In the present study, an ultra performance liqu...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhou WJ,Song JZ,Fu WW,Tan HS,Bian ZX,Xu HX

更新日期：2013-10-01 00:00:00

abstract：:Benzodiazepines are among the most frequently prescribed drugs due to their sedative, hypnotic, anxiolytic, muscle relaxant and antiepileptic properties. Considering the high consumption of benzodiazepines worldwide, there is increased potential for addiction and abuse in cases of crime, driving under the influence of...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Bertol E,Vaiano F,Furlanetto S,Mari F

更新日期：2013-10-01 00:00:00

abstract：:To evaluate the quality consistency of commercial medicinal herbs, a simple and reliable HPLC method with UV-vis detector was developed, both for fingerprint analysis and quantitation of some pharmacologically active constituents (marker compounds). Melissa officinalis L. (lemon balm) was chosen for this study because...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Arceusz A,Wesolowski M

更新日期：2013-09-01 00:00:00

abstract：:A new stability-indicating method based on high-performance liquid chromatography coupled to ultraviolet and evaporative light scattering detection (HPLC-UV-ELSD) was developed for the quantification of daunorubicin. This is an ion-pairing, reversed-phase method. The column was a Synergi MAX-RP C12 4 μm (150 mm × 4.6 ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Respaud R,Quenum L,Plichon C,Tournamille JF,Gyan E,Antier D,Viaud-Massuard MC

更新日期：2013-09-01 00:00:00

abstract：:Polygonum jucundum is a traditional Chinese medicine used for the treatment of diseases. The major bioactive compound in the ethanol extract of P. jucundum is 2α-hydroxyl-3β-angeloylcinnamolide (HAC), a drimane-type sesquiterpenoid, which possesses anti-inflammatory activities. The purpose of this study was to investi...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Zhang F,Gong X,Xiao B,Zhang C,Wang Z

更新日期：2013-09-01 00:00:00

abstract：:The purpose of this study was to investigate the in vitro release of 5-aminosalicylic acid from single extrudates by UV imaging and to explore the technique as a visualization tool for detecting film coating defects on extrudates coated with a thin ethyl cellulose layer. 5-Aminosalicylic acid extrudates were film coat...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Gaunø MH,Vilhelmsen T,Larsen CC,Boetker JP,Wittendorff J,Rantanen J,Østergaard J

更新日期：2013-09-01 00:00:00

abstract：:This study demonstrates the use of hydrophilic interaction liquid chromatography with a nano quantity analyte detector for the retention, separation and detection of magnesium from magnesium stearate in tablet formulations for a drug product formulation blend containing a hydrochloride salt of a weakly basic compound ...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Risley DS,Magnusson LE,Morow PR,Aburub A

更新日期：2013-05-05 00:00:00

abstract：:Aim of this work was to gain a deeper insight into the analytical profile of the macromolecular and LMW fractions of polymeric silicones present in breast implants. The study was conducted on silicone gel samples from (i) breast prostheses (Poly Implant Prothèse, PIP) explanted from a patient that needed their therape...

journal_title：Journal of pharmaceutical and biomedical analysis

authors： Beretta G,Malacco M

更新日期：2013-05-05 00:00:00